How to reconstitute NAD+ for research
A step-by-step guide to reconstituting lyophilised NAD+ with bacteriostatic water, with the concentration maths.
Buy NAD+ 500 mg · Nicotinamide adenine dinucleotide
Reconstituting NAD+ step by step
NAD+ is supplied as a lyophilised (freeze-dried) powder and is reconstituted with bacteriostatic water for research. The volume of water you add sets the concentration.
1. Work out your target concentration
Decide how much peptide you want per unit of the solution. The vial contains 500 mg. Adding more water gives a lower concentration and more total volume; less water gives a higher concentration.
2. Add bacteriostatic water slowly
Run the water down the inside wall of the vial rather than directly onto the powder, and let it dissolve without shaking. Swirl gently if needed.
3. Calculate your units
Use the reconstitution calculator — enter the vial strength (500 mg), the water volume and your target, and it returns the units, millilitres and concentration.
4. Store the reconstituted vial
Keep the reconstituted vial refrigerated and protected from light. Work to good laboratory practice.
Frequently asked questions
Is NAD+ for human use?
No. NAD+ is supplied strictly for laboratory and in-vitro research use only.
How is NAD+ stored?
Store sealed vials at −20 °C, dry and out of light; keep the reconstituted vial refrigerated.
Where can I buy NAD+ in Australia?
You can buy NAD+ from StarTide with express post Australia-wide.